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Image Search Results
Journal: Pharmaceutical Biology
Article Title: Cardamonin suppressed the migration, invasion, epithelial mesenchymal transition (EMT) and lung metastasis of colorectal cancer cells by down-regulating ADRB2 expression
doi: 10.1080/13880209.2022.2069823
Figure Lengend Snippet: CDN suppressed ADRB2 expression and viability of CRC cells through inhibiting ADRB2 expression. Cells transfected with siADRB2 or ADRB2 overexpression plasmid were exposed to CDN and then examined for the mRNA level of ADRB2 and the viability using RT-qPCR (A, B) and CCK-8 (C, D), respectively. GAPDH acted as the internal control. ** p < 0.01 or *** p < 0.001 vs. NC; ## p < 0.01 or ### p < 0.001 vs. NC + CDN; ^^^ p < 0.001 vs. ADRB2; ++ p < 0.01 or +++ p < 0.001 vs. siNC; && p < 0.01 vs. NC + CDN-10; △△△ p < 0.001 vs. siADRB2. NC: negative control (empty vector); siNC: negative control of small interfering RNA (siRNA); CDN: cardamonin; CRC: colorectal cancer; ADRB2: β 2 adrenergic receptor; CCK-8: cell counting kit-8; RT-qPCR: reverse transcription-quantitative polymerase chain reaction; siADRB2: siRNA for ADRB2.
Article Snippet: SiADRB2 (siG000000154A-1-5) and
Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation, Quantitative RT-PCR, CCK-8 Assay, Control, Negative Control, Small Interfering RNA, Cell Counting, Reverse Transcription, Real-time Polymerase Chain Reaction
Journal: Pharmaceutical Biology
Article Title: Cardamonin suppressed the migration, invasion, epithelial mesenchymal transition (EMT) and lung metastasis of colorectal cancer cells by down-regulating ADRB2 expression
doi: 10.1080/13880209.2022.2069823
Figure Lengend Snippet: CDN blocked migration and invasion of CRC cells through inhibiting ADRB2 expression. Cells transfected with siADRB2 or ADRB2 overexpression plasmid were exposed to CDN and then examined for the migration and the invasion by wound healing assay (A, B, E and F) and transwell assay (C, D, G and H), respectively. *** p < 0.001 vs. NC; ## p < 0.01 or ### p < 0.001 vs. NC + CDN; ^^ p < 0.05 or ^^^ p < 0.001 vs. ADRB2; + p < 0.05 or ++ p < 0.01 or +++ p < 0.001 vs. siNC; &&& p < 0.001 vs. NC + CDN-10; △△△ p < 0.001 vs. siADRB2. NC: negative control (empty vector); siNC: negative control of small interfering RNA (siRNA); CDN: cardamonin; CRC: colorectal cancer; ADRB2: β 2 adrenergic receptor; siADRB2: small interfering RNA for ADRB2.
Article Snippet: SiADRB2 (siG000000154A-1-5) and
Techniques: Migration, Expressing, Transfection, Over Expression, Plasmid Preparation, Wound Healing Assay, Transwell Assay, Negative Control, Small Interfering RNA
Journal: Pharmaceutical Biology
Article Title: Cardamonin suppressed the migration, invasion, epithelial mesenchymal transition (EMT) and lung metastasis of colorectal cancer cells by down-regulating ADRB2 expression
doi: 10.1080/13880209.2022.2069823
Figure Lengend Snippet: CDN lessened MMP-2, MMP-9 and N-cadherin expression but augmented E-cadherin expression in CRC cells through inhibiting ADRB2 expression. (A–F) ADRB2 overexpression or knockdown was achieved by transfection of ADRB2 overexpression plasmid or siADRB2 into CRC cells, respectively. Transfected cells were exposed to CDN and then examined for the mRNA and protein levels of MMP-2, MMP-9, E-cadherin and N-cadherin using RT-qPCR (A, D) and Western blotting (B, C, E and F). GAPDH acted as the internal control. *** p < 0.001 vs. NC; ## p < 0.01 or ### p < 0.001 vs. NC + CDN; ^^ p < 0.05 or ^^^ p < 0.001 vs. ADRB2; + p < 0.05 or ++ p < 0.01 or +++ p < 0.001 vs. siNC; && p < 0.05 or &&& p < 0.001 vs. NC + CDN-10; △△△ p < 0.001 vs. siADRB2. NC: negative control (empty vector); siNC: negative control of small interfering RNA (siRNA); CDN: cardamonin; CRC: colorectal cancer; ADRB2: β 2 adrenergic receptor; RT-qPCR: reverse transcription-quantitative polymerase chain reaction; MMP: matrix metalloprotease; siADRB2: small interfering RNA for ADRB2.
Article Snippet: SiADRB2 (siG000000154A-1-5) and
Techniques: Expressing, Over Expression, Knockdown, Transfection, Plasmid Preparation, Quantitative RT-PCR, Western Blot, Control, Negative Control, Small Interfering RNA, Reverse Transcription, Real-time Polymerase Chain Reaction